International Journal of Environment (ISSN: 2186-0009)  


Phenol Biodegradation by Two Strains of Pseudomonas putida and Effect of Lead and Zinc on the Degradation Process
Abdullah-Al-Mahin, Alamgir Z Chowdhury, Md. Khorshed Alam, Zakia Aktar and ANM Fakhruddin
Abstract:
In this study, biodegradation of phenol by Pseudomonas putida strains A(a) and CP1 and the effect of lead and zinc on the biodegradation were investigated. A(a) and CP1 completely degraded phenol up to 1000 mg/l and 800 mg/l, respectively. In the presence of phenol as the sole carbon source, the removal rate was higher by A(a) than CP1, however, when 0.5% glucose was added, the rate was higher by CP1. Growth yields of the strains were increased with increasing concentrations of phenol. Although flocculation was detected when cells of A(a) and CP1 were grown in ≥ 800 mg/l and ≥ 400 mg/l phenol, respectively, this phenomenon was absent with additional presence of 0.5% (w/v) glucose in 500 mg/l phenol for both strains. Moreover, although A(a) could degrade 400 mg/l phenol in presence of either lead or zinc in a concentration of up to 2.0 mM the degradation was slowed down.
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